�d��5�ֹܵܯ�y!%r%��]iHg�3�g����U}�%�^u]}�l���Y{��������S}}����v��u���q�������""�trv���H2�O$F%�1�)���ϟe�5��ϟ�ϒ�o�ٿ�? It represents one aspect of water quality. 5.2.1 Pour Plate Method Dispense one ml of sample into two Petri dishes. Surface water sampling methods and analysis — technical appendices Standard operating procedures for water sampling- methods and analysis Looking after all our water needs Department of Water … 4.1.16 Again collect the swab sample and repeat procedure following Point 4.1.1 to 4.1.13. Wrap the neck with aluminum foil to avoid any contamination during transportation. This document is not intended for public release. <> Please select the appropriate link in the table below to send your request via email, or contact Health Canada's publication office to make your request.. <> Standard Operating Procedure (SOP) for sampling from different points and microbiological analysis/testing of Purified water samples in pharmaceutical plants. Use sodium hypochlorite solution (1g/L free chlorine) or ethanol/isopropanol 70% v/v. Carry the sampling containers to the sampling point as per the sampling schedule. The Laboratory Procedures for the Microbiological Analysis of Foods are available upon request. MICROBIOLOGICAL WATER SAMPLING - BASIC PROCEDURES Taking a Routine Bacteria Sample Properly Step One Do Not Rinse The Bottle The powder in the bottle is meant to be there and will not contaminate your sample. Analyse the water samples for Microbiological analysis as per specifications. WATER ANALYSIS SAMPLING PROCEDURES Microbiological samples: Microbiological samples should be collected in sterile plastic or glass bottles which Vinlab supplies. 3. Sampling apparatus and containers shall be clean, sterile, and/or endotoxin-free as per test requirements. SUPPLY … PROCEDURE: Sampling of water Sampling of water … Perform the analysis as per the current version of purified water specification. Sanitize the hands with 70 % v/v IPA. OBJECTIVE:To lay down a procedure for microbiological monitoring of raw water and purified water. Alert levels are always lower than Action levels. Microbiological Analysis of Water Quality •Looking in to the 21st Century sigma-aldrich.com January 31, 2012 By Richard Glindon Presented by Klaus Herick. Refer Annexure-2 for preparing the Sampling Schedule. • In systems with more than one water source, the locations of the sampling points should take account of the number of inhabitants served by each source. Microbiologist shall wear sterilised gloves during the sampling activity. Then remove the sterile strip from the bottle. These stored samples should be analyzed within 6 hours. Microbiological samples should be collected in sterile plastic or glass bottles. Ensure that the required contact time for the sanitizer has been achieved. Talk to the lab to determine if this collection procedure is acceptable for the analytical method they plan to perform. 5.1.4 For a sampling of Treated Raw water (May contain chlorine), add 0.1 ml of 5 % sodium thiosulphate solution in the bottles before sterilization. Write the sampling details or affix the label on the bottle as per Annexure-3. 2. Faecal contamination of water is routinely detected by microbiological analysis. Step Two Wash Your Hands prior to taking the sample. Phase 2 requires a root cause analysis and corrective action. Moreover, there are many types of pathogen and each requires a unique microbiological isolation technique. Sampling Procedures for Drinking Waters 1 PURPOSE This standard operating procedure outlines details for collection of drinking water samples for chemical, microbiological and radiological analysis. Bacteriological water analysis is a method of analysing water to estimate the numbers of bacteria present and, if needed, to find out what sort of bacteria they are. 1. Before analysis, Register/log the sample, and generate/issue the worksheet, or the template. Approximately add 15-20 ml of R2A / Plate count Agar into each Petri dishes. 5.1.3 Samples for microbiological evaluation should be collected in Pre-sterilized screw cap glass bottles. Annexure-1: List of Sampling Points of Purified Water. 4.12 Control the valve of sampling point to avoid splashing. Close the container immediately and record the sampling time. Sampling personnel shall wear clean smocks or gowns and sterile gloves prior to collecting any sample (As per area requirements). ISO 19458:2006 provides guidance on planning water sampling regimes, on sampling procedures for microbiological analysis and on transport, handling and storage of samples until analysis begins. Open tap and run to waste, allowing removal of any excess solution. Open the valve of the sampling point and allow the water to drain for about 20-30 seconds. If a hose is not present, a sterilized adapter and hose may be used to sample directly from the valve/port. Head QA shall be responsible for the approval of SOP. Hold the sterile container near the sampling point. Noroviruses, are repeatedly related to poor water quality (… SAMPLING FOR MICROBIOLOGICAL ANALYSIS In case of laboratory analysis for microbiological purpose, following guidelines are adopted to collect representative water samples: Sample Bottles Pre-sterilized sampling bottles of 200 ml capacity made of pre-sterilized disposible/autoclavable plastic, or good quality soda / borosilicate glass. ACoP L8 recommends that sampling for Legionella should be carried out when: Unwrap the aluminum foil and open the bottle taking care not to touch the inner surface and neck of the bottle. <>/XObject<>/ProcSet[/PDF/Text/ImageB/ImageC/ImageI] >>/MediaBox[ 0 0 612 792] /Contents 4 0 R/Group<>/Tabs/S/StructParents 0>> Alert levels are specific for a given facility and are established on the basis of a baseline developed under an activity monitoring program. The precautions taken to avoid contamination must be such that they do not affect any microorganisms that are to be revealed in the test (water analysis). endobj Rinse the container at least one to two times with purified water from the sampling point. Start microbiological analysis of water as soon as possible after collection to after sampling and certainly within 24 hours. Close the bottle with a cap and wrap the neck with aluminum foil and Sterilize the bottle at 121 ºC (15 lbs.) Correct sampling procedures as instructed by international standard protocols must be adhered to; Routine microbiological testing of drinking water supplies, recreational waters, and environmental waters is essential for the protection of public health ; Programme of testing. If any chemical parameter exceeds the limit, immediately inform the same QA, Production, and Engineering Department. Rinse with purified water. Wash 125 ml Amber glass or suitable bottles with a stopper with 2.0 M HCL or 2.0% nitric acid and rinse with purified water and dry the bottles at 105 ºC for at least one hour. It is important to routinely identify organisms recovered from water systems when: Waterborne organisms may be detrimental to finished products or processes in which the water is used. 4 0 obj Water system sanitization methods require assessment. SCOPE:This SOP shall provide the procedure for sampling and testing of raw water and purified water from all the user points and all the points across the critical functions in the water purification process. 2 Content • Introduction – What does the future hold for microbiological water AQC? Prepare a Sampling and Analysis Plan (SAP) which describes the sampling locations, numbers and types of samples to be endobj Preparation of Containers for the sampling of purified water: Clean 1000 ml Clear glass bottles with a cap. • There should be at least one sampling point directly after the clean-water Aseptic Technique for Microbiological Testing. Domestic water systems are supplied with water fit to drink, so ACoP L8 advices that regular microbiological sampling is not required. Note: Before sampling, the aluminum foil covering the sampling point should be removed. Flush the sample port/valve for the qualified time or volume and immediately open the labeled sample container and collect the sample. Analyze the sample of purified water for pathogens using filtration technique/Direct as per the current specification of Purified water. • Sampling points should be located in such a way that water can be sampled from reserve tanks and reservoirs, etc. – How can we meet the demands microbiological water AQC in the future? 4.2 Rinse water sampling: 4.2.1 After cleaning, the Production Officer shall inform to QA Officer for the collection of the rinse water sample. Close the bottle with a cap and wrap the neck with aluminum foil and Sterilize the bottle at 121 ºC (15 lbs.) Collect the 100ml of water sample for self analysis and 500ml of water for outside lab for microbiological analysis … {cy�L���*3i��S��e�T�����4�VS�H�¤y��2��d�����4(�"^�xnfg���kS��p�'�T�@�ů�_�:�}��$w��>�&���=������o3!�J� �H�bZ� 3. Water Sampling Procedure: 1. Close the valve and bring the samples to the laboratory and make an entry in the purified water inward register. Quality Monitoring of Water for Pharmaceutical Use. Sampling Schedule of Purified Water: In particular, the spread of enteric viruses, e.g. Wear gloves and nose mask before sampling. Water quality — Sampling for microbiological analysis. Carefully replace the cap. <>>> Carry out the chemical analysis of samples as per the current version of purified water specification. Ensure you have read the ‘Standard drinking water sampling procedure – microbiological’. Sampling Instructions This method of sample collection is acceptable for EPA analytical methods 1622 and 1623 when sending water samples in to the laboratory without filtering in the field. Phase 2 follows the determination of data validity and investigates the water system and all potentially affected lots of products. Water Sampling Procedure (with reference to ISO 5667 Part 5) (A) Assessment of Sampling Location Please take note of the conditions of the environment, sampling taps and pipes at the sampling location. Collect the purified water sample (refer table 1) without rinsing and close it with stopper immediately. Wipe any visible dust from the exterior of the sample port or valve using a lint-free wipe/cloth moistened with an approved sanitizer – do not spray or wipe the interior of the port or valve. Immediate resampling of the specific location where the OOT/OOS occurred must be performed, to rapidly assess the condition of the system. If a hose is already connected to the port or valve, collect the sample directly from that hose. 4.2.2 Enter into the respective area and visually observe the machine or part of the machine. Replace the used hose onto the provided clamp after sampling so that it fully drains. 1 minute. A designated person shall issue the template/worksheet. Head of the Department / Designee of respective areas & QA shall be responsible for reviewing the SOPs. Analyze the sample of purified water for Total Viable Count using filtration technique as per the current specification of Purified Water. For Microbiological Analysis: Clean 250 ml Clear glass bottles with cap for membrane filtration method or pour plate method and rinse with purified water. for validated time. Rinse the bag twice with the sample water prior to filling and closing. endobj 4.11 Open the valve of sampling point slowly to full and drain water for approx. If you are sampling more than one location you need a separate sample bottle for each location. Chemical analysis: Vinlab supplies 100ml sterile plastic bottles. OBJECTIVE To lay down a procedure for microbiological monitoring of raw water and purified water SCOPE: This SOP shall be applicable for sampling and microbial analysis of raw and purified water from all user points. 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